Synergistic effects of sequential treatment by Ultraviolet irradiation and chemical disinfectant
for drinking water disinfection.
The sequential use of disinfection strategies for drinking water treatments has been shown to provide levels of
inactivation greater than those corresponding to the single-step disinfection effects (Corona-Vasquez et al., 2002;
EPA, 1999). Therefore, a disinfectants combined system could be applied both for the inactivation of microorganisms,
resistant to traditional treatments, and also for limiting the dosage of single disinfectants, reducing
cost and risk of by-products formations. In this work, the inactivation of the bacteria E. coli was initially
investigated by sequential action of Ultraviolet irradiation (bench-scale collimated beam apparatus equipped with a low
pressure Ultraviolet lamp) and either chlorine dioxide or free chlorine. The results showed that a pre-irradiation of 70
J.m-2 Ultraviolet dose (equal to approximately 1.2 log of inactivation) at 20 0C and pH 7.4, increased the following
germicidal effect of both chlorine and chlorine dioxide (between 0.04 and 1 mg min L-1). A different order of the
disinfectants was also studied and a similar performance was observed. In all cases, an enhanced signal was
observed and an inactivation of approximately 1 log greater than that seen in the inactivation curve for free
chlorine and chlorine dioxide alone was achieved. An in-situ application of the sequential disinfection system
consisted in a combined use of Ultraviolet irradiation and chlorination to eliminate a saprophyte flora interfering during
the faecal contamination indicator analyses. Likewise, the outlet of a reservoir for drinking water has been firstly
disinfected by chlorine dioxide and then irradiated by Ultraviolet. In both cases, an increase of the microbiological
quality of the water was achieved, compared to the quality observed with a single step disinfection. This
observation led to more bench scale investigations concerning the potential synergistic effects of Ultraviolet associated
with chemical oxidants for water disinfection. In particular, preliminary studies were addresses to synergistic
action for virus MS-2 bacteriophages inactivation.
Key words: Disinfection, Ultraviolet Irradiation, Synergy, Chlorine, E. coli.
In the last decades, the synergy of different disinfectants for drinking water treatments has interested a certain
number of scientists in the world. The use of a disinfectant agents combination was adopted mainly in cases
where the single use disinfectant (free or combined chlorine as well as ozone) was not effective for specific
micro-organisms disinfection (e.g. Cryptosporidium parvum) (Finch et al., 1997). Only recently, the Ultraviolet
irradiation was discovered to be an optimal disinfectant for Cryptosporidium parvum, at relatively low doses
(Clancy, 2000). Nevertheless the synergetic action of several disinfectant is investigated and the combination
of the Ultraviolet irradiation with other disinfectants is considered of great interest. By carefully selecting the primary
and secondary disinfectant and avoiding long contact times and high dosages, the total DBP formation has been
shown to decline (EPA, 1999). Although the mechanisms of action by which the combination of two
disinfectants increases the disinfection action is still not clear, several studies have been published (Rennecker et
al., 2000). Most of the work concerns the combination of ozone with free and combined chlorine, few paper are
reported on Ultraviolet irradiation combined to other disinfectants.
A comparison of single use disinfectants (Ultraviolet, free chlorine and monochloramine) and the combined use of Ultraviolet / free chlorine and Ultraviolet / monochloramine for Cryptosporidium parvum inactivation was presented by Ramirez
(Ramirez et al., 2000). Cryptosporidium parvum oocysts were pre-treated with Ultraviolet light at a dose of
approximately 1250 J/cm2 (primary inactivation of approximately 1 log) and exposed to monochloramine. The
shoulder of the secondary inactivation curve was shorter and the post-shoulder rate of inactivation was
approximately the same as that observed for primary treatment with monochloramine. When the secondary
disinfectant was free chlorine, the exposure to Ultraviolet light resulted in a decrease in the secondary lag-phase and a
two-fold increase in the rate of inactivation in the post –lag phase pseudo first order region.
Shin (Shin et al., 2002) studied the kinetics and levels of inactivation of several important waterborne
pathogens by various doses of monochromatic, low-pressure (LP) Ultraviolet lamps, followed by free and combined
chlorine. The results of this work indicated that there was no apparent evidence of synergism between LP-Ultraviolet
and free chlorine for C. parvum oocysts and Adenovirus 5 inactivation, under the condition tested. However,
there appeared to be some effect of prior Ultraviolet irradiation on inactivation of a genetically modified strain of S.
typhimurium by 1 mg/l of monochloramine. More controlled laboratory studies are necessary to confirm the
potential advantages of a preventive use of Ultraviolet associated to others disinfectants, to design new sequential
disinfection systems for large scale implementations and drinking water utilities.
Material and Methods
Cultures of Escherichia coli (strain RIVMWR 1), stored in 50 % glycerol at – 20 0C, were used for inoculation
onto a trypto casein soya (TCS) solution. After overnight (16 h) incubation at 37 0C, an inoculum of 10 µl was
added to 10 ml of fresh TCS at 37 0C for other 16 hours of incubation (corresponding to the late exponential
phase of growth). The culture was then harvested by centrifugation for 5 minutes (4 rpm), and the pellet resuspended
in 10 mM phosphate buffered saline (PBS) at pH 7.4 (2.7 mM KCl, 137 mM NaCl), after two washes
in the same buffer and re-homogenisation, using a vortex mixer. The stock was diluted in PBS to a working
concentration of 106 /ml. The E. coli stock suspension concentration was determined by pour plate method and
by optical density (OD) at 578 nm. A calibration curve of the absorbance in function of the bacteria
concentration was obtained. The concentration of E. coli was checked before each experiment by absorbance
A stock suspension of MS-2 bacteriophages was stored at –20 0C at a concentration of 3*1011 /ml. The stock was
diluted in PBS to achieve a concentration of 3*107 /ml.
Inactivation of micro-organisms by Ultraviolet irradiation
The effect of ultraviolet irradiation on E. coli was investigated at bench scale, using a 254 nm collimated beam
apparatus (Calgon Carbon). It consists of one 8 Watt low pressure (LP) mercury vapor germicidal lamp emitting
nearly monochromatic Ultraviolet radiation at 253.7 nm. Ultraviolet irradiance at 253.7 nm was measured with a radiometer
IL1700. The irradiation dose was calculated using a calculation file for dose-response curve programmed by"Bolton" (519-741-6283) which takes into consideration all the parameters playing a role in the irradiation of a
water samples. The suspension was kept under stirring during all the irradiation time, at room temperature. The
proportion of surviving organisms was detected by pour plate method and plotted against Ultraviolet dose. A non
irradiated control sample was always considered.
Production of chlorine dioxide and free chlorine
Chlorine dioxide was obtained by mixing in very little amount diluted solution of sodium chlorite (7.5%: from
stock, 0.8 g in 10 ml of water Millipore Ultrapure) and chloride acid (9%: HCl from stock 35%, 1:4 dilution of
35% HCl in water Millipore Ultrapure). In a 15 ml polyethylene container, 7.5% NaClO2 (2.5 ml ) was added
first, followed by 9 % HCl (2.5 ml). The reacting solution was mixed very gently and left still for about 10
minutes, in the dark. After filling with water, the bottle content was transferred in a brown glass bottle (250 ml),
containing 150 ml of water and filled to a final volume of 250 ml. The chlorine dioxide concentrations was
determined by direct light spectro-photometry (reading absorbance at 360 nm, molar absorbivity = 1250 M-1 cm-1). Household bench (2.6 % active chlorine, Javel) was diluted in chlorine demand free water to prepare a 2.5
mg/L stock solution. Working solutions of free chlorine at target concentrations was prepared in same water
prior to experiments. The yield was analysed using the N, N diethyl-p-phenylenediamine (DPD) colorimetric
Sequential disinfection by Ultraviolet and chlorine dioxide /free chlorine
A Ultraviolet dose (50-70 J/m2) necessary to achieve 1 log inactivation was applied before, after and together with ClO2
concentrations for E.coli inactivation, whilst a pre-Ultraviolet treatment was carried out for E.coli inactivation with
HClO as a secondary disinfectant and for MS-2 inactivation with ClO2 as a secondary disinfectant. A set of
samples were treated in the same range of ClO2/ HClO concentrations but without Ultraviolet irradiation. A non treated
control was always kept in the same condition (pH, temperature) than those of the other samples. The chemical
reaction with ClO2 or HClO was stopped by adding sodium thiosulphate, after 30 seconds.
The viable concentration of E.coli and bacteriophages MS-2 was achieved after surface counting plate
enumeration and the double agar layer method respectively. The number colony forming units for E. coli and
plaque forming units for MS-2 were counted after an 18-24 hours incubation period at 37 0C. The synergy appears when the combination of agents is more effective than the expected single components
effect. The proportion of surviving micro-organisms (expressed as Log N/N0) was plotted against ClO2 or HClO
concentrations. The synergy of the sequential disinfection was calculated as:
Results and Discussion
The inactivation curves for E. coli by pre-Ultraviolet irradiation at 50 - 70 J/m2 and ClO2, in a range of concentrations of
0.05-1 mg/L are shown in Figure 1.
The treatment with ClO2 alone was characterised by a curve with a shoulder region followed by a decrease in
viability, whilst no shoulder was observed after the Ultraviolet pre-treatment. Moreover, a rate of inactivation post
shoulder was enhanced in the case of combined disinfection for a concentration of chlorine dioxide of 0.4 mg/L
min. A synergistic effect was obtained with peaks of 0.91 and 1.42 (70 J/m2 and 50 J/m2 respectively) for ClO2
of 0.4 mg/L min. When the Ultraviolet was used as a secondary disinfectant (Figure 2), a similar enhanced inactivation
was observed and a synergistic effect was achieved with a maximum of 1.2 log for 0.20 mg/L min. In this case, a
decrease of synergy was observed by increasing the concentrations of chemical disinfectant.
The inactivation of E.coli was also observed after simultaneous disinfectant action of Ultraviolet (70 J/m2) and ClO2
(0.02-1 mg/L min) (Figure 3). When a CT= 0.2 mg/L min was applied, an inactivation of 4 logs was achieved for
the combined disinfection, whereas the only chlorine dioxide gave less than 1 log inactivation. An enhanced
inactivation of 1.47 log due to the synergistic effect was observed. Also, the dosage of chlorine dioxide seemed
to increase as the synergistic effect decreased.
Since the different order does not seem to be responsible for important improvements of synergy, it was decided
to use Ultraviolet irradiation as first disinfectant for having the chemical agents as secondary disinfectant. This provides
a residual in the distribution systems, thus a minor risk of undesired by-products, due to the Ultraviolet action in the
presence of the chemical disinfectants or other water components. Likewise, the synergistic effect of Ultraviolet
combined to HClO was tested and the inactivation curve for E. coli disinfection is presented in Figure 4.
A very little inactivation was observed with free chlorine alone. The pre-Ultraviolet disinfection treatment gave a
synergy of 0.8 log for CT= 0.1 mg/L min. Further studies need to be carried for this synergetic disinfection
system. The synergy observed for all experiments is summarised in table 1.
A similar approach was carried out for inactivation of MS-2 bacteriophages by pre-irradiation with a dose of 200
J/m2, followed by ClO2 (CT= 0.05-0.5 mg/L min) (Figure 5). In this case there was no apparent evidence of
synergism between Ultraviolet irradiation and chlorine dioxide for MS-2 inactivation. Even in absence of synergy, the
application of the additional disinfectants allows a considerable inactivation of resistant micro-organisms such as
Sequential disinfection using Ultraviolet combined disinfection system has been applied to different water plants. A first
disinfection process based on combined ClO2 / Ultraviolet action has been used for a drinking water plant close to
Toulouse (France)(Esparza, 1997). The disinfection procedure was initially carried on with chlorine dioxide. The
water was then delivered to a reservoir where, before the distribution, a new disinfection step was realised with
Ultraviolet. The curves in Figure 6 show a high inactivation of germs (the heterotrophic plate count (HPC) was applied
for micro-organism measurements, at both 22 0C and 33 0C) during the weeks of the disinfection treatment. A
good disinfection was obtained in terms of germs inactivation.(lower than 2/ml after disinfection treatment) and
a low consumption of chemicals was also guaranteed (Esparza, 1997).
Another example of sequential disinfection on field application concerns the elimination of a saprophyte flora on
a spring close to Deauville (France). The presence of a saprophyte flora (SF) provoked interference during the
E.coli analyses (TTC Tergitol, pour-plate counting method). Despite an initial disinfection step with gaseous
chlorine, the saprophyte flora was still present in high concentration. The following application of Ultraviolet (via a low
pressure lamp), at a dose up to 1000 J.m-2 did not allow a sufficient elimination of the interference neither. The sequential disinfection Ultraviolet / Cl2 showed a very good result in comparison with the results obtained with the
individual treatments as it is presented on the Figures 7 and 8.
Figures 7 and 8 showed that even if the chlorination and the Ultraviolet irradiation alone could not provide a sufficient
disinfection, the combination of the two processes allowed to reach the objectives, without risk of a by-products
formation. These results are in agreement with the data observed in the laboratory experiments on E. coli.
A good synergy was observed for E.coli inactivation after Ultraviolet / ClO2 treatment during the bench scale
experiments, independently from the disinfectants order. The synergy effect was of 1.4 log inactivation for range
0.1-0.5 mg/L min of ClO2 The use of Ultraviolet as primary disinfectant resulted in removal of the shoulder region for
secondary disinfection. Synergistic effect was observed for E.coli inactivation after Ultraviolet / HClO treatment with a
rate of inactivation up to 0.8 log for 0.1 mg/L min. Even in absence of synergy, the application of the additional
disinfectants can allow a considerable inactivation of the micro-organisms. Examples of sequential disinfection
for larger implementations are reported and, despite the more complex experimental conditions, the results were
in agreement with the laboratory results. A preventive use of Ultraviolet would be envisaged since it reduces the risk of
harmful disinfection by-products and it is effective for inactivating resistant micro-organisms. Other
disinfectants (e.g. ozone) combined with Ultraviolet should be considered.
Clancy, J.L. (2000). Ultraviolet light inactivation of Cryptosporidium. IOA, Berlin, Oct. 23-26 III.2.1-III.2.14.
Corona-Vasquez, B., et al. (2002). Sequential inactivation of Cryptosporidium parvum oocysts with chlorine
dioxide followed by free chlorine or monochloramine. Water Research. 36, 178-188.
EPA. (1999). Alternative disinfectants and oxidants. EPA, April 1999. Guidance Manual, Combined
Esparza, A. (1997). La technique de desinfection par U.V. appliquee a la production d'eau potable a Toulouse.
L'eau, l'Industrie, les Nuisances. 205, 45-47.
Finch, G.B., et al. (1997). Effect of various disinfection methods on the inactivation of Cryptosporidium.
AWWA Research Foundation Report, Denver, USA.
Li, H.B., et al. (2001). Chlorine dioxide inactivation of Cryptosporidium parvum in oxidant demand-free
phosphate buffer. Journal of Environmental Engineering-Asce. 127, 594-603.
Ramirez, L.M., et al. (2000). Ultraviolet disinfection of C. Parvum oocysts in a batch reactor. Awwa. Water Quality
Technology Conference, Salt Lake City, UT, USA
Rennecker, J.L., et al. (2000). Synergy in sequential inactivation of Cryptosporidium parvum with ozone/free
chlorine and ozone/monochloramine. Water Research. 34, 4121-4130.
Shin, G.A., et al. (2002). Sequential disinfection with Ultraviolet irradiation and chlorine species on several important
waterborne pathogens. Awwa. Water Quality Technology Conference, Seattle, WA, USA.